设为首页 |加入收藏 |联系我们 |ENGLISH

 

首页

 

期刊概况

 

编委会

 

过刊浏览

 

特邀审稿

 

投稿指南

 

出版发行

 

联系我们

FMN2 在胃腺癌组织中的表达及其临床意义[J].贺奋飞,余彦平,李云龙,赵昕辉,王珂,陈文平,李纪鹏.中国肿瘤生物治疗杂志,2018,(9):928~933.
FMN2 在胃腺癌组织中的表达及其临床意义    点此下载全文    点此浏览HTML全文
贺奋飞  余彦平  李云龙  赵昕辉  王珂  陈文平  李纪鹏
1.空军军医大学第一附属医院 消化外科,陕西 西安710032;2.西安医学院研究生处,陕西西安710068;3. 西安马应龙肛肠医院,陕西西安710005
基金项目:陕西省社会发展与攻关基金资助项目(No. 2014SF2-10)
DOI:10.3872/j.issn.1007-385X.2018.09.013
摘要:
      目的:检测成蛋白2(formin-2,FMN2)在胃腺癌组织中的表达水平,探讨FMN2 的表达与胃癌患者临床特征之间的关系及其对胃腺癌AGS细胞增殖的影响。方法:收集2015 年9 月至2017 年9 月空军军医大学第一附属医院消化病医院胃肠外科手术切除的胃腺癌组织及相应的癌旁组织标本84 例。采用免疫组织化学染色和肿瘤数据库检索检测分析胃腺癌组织中FMN2的表达水平,并探讨FMN2 蛋白表达与胃腺癌患者临床病理特征之间的相关性;MTT法检测FMN2 对胃腺癌AGS细胞增殖活性的影响,Western blotting 法检测FMN2 对胃腺癌AGS细胞凋亡蛋白Caspase-3 表达的影响。结果:胃腺癌组织中FMN2 表达水平明显低于癌旁组织(P<0.05 或P<0.05),其低表达与胃腺癌患者的TNM分期及肿瘤分化程度相关(均P<0.05);与AGS细胞对照比较,AGS/FMN2 稳转株的增殖率明显降低、凋亡相关蛋白Caspase-3 表达明显增加(均P<0.05)。结论:FMN2 在胃腺癌组织中表达明显下调,且与患者的肿瘤分化程度和TNM分期相关;过表达FMN2 的胃腺癌细胞其增殖率明显降低;FMN2 是胃癌预后的独立危险因素,可为胃腺癌的治疗提供新的思路。
关键词:胃腺癌  成蛋白2  AGS细胞  增殖  凋亡
Expression of FMN2 in gastric adenocarcinoma tissues and its clinical significance    Download Fulltext
HE Fenfei  YU Yanping  LI Yunlong  ZHAO Xinhui  WANG Ke  CHEN Wenping  LI Jipeng
1.Gastroenterology surgery, the First Affiliated Hospital of Air Force Military Medical University, Xi’an 710032, Shaanxi, China; 2. Graduate office,Xi’an Medical University,Xi’an 710068, Shaanxi, China; 3. Xi'an Ma Ying Long Anorectal Hospital, Xi’an 710005, Shaanxi, China
Fund Project:Project supported by the Social Development and Tackle Key Problems Fund of Shanxi Province(No. 2014SF2-10)
Abstract:
      Objective: To investigate the expression of Formin-2(FMN2)protein in gastric cancer tissues and its correlation to clinicopathological features of gastric cancer patients, as well to explore its effect on the proliferation of gastric adenocarcinoma AGS cells.Methods: 84 cases of gastric adenocarcinoma tissues and corresponding para-cancerous tissues were surgically collected from patients treated in the First Affiliated Hospital of Air Force Military Medical University from September 2015 to September 2017. The expression of FMN2 in gastric adenocarcinoma tissues was detected by immunohistochemical staining and analyzed with RNA-Seq data-sets GEPIA. The relationship between FMN2 protein expression in gastric adenocarcinoma tissues and its clinicopathological features was also explored. MTT assay was used to detect the effect of FMN2 on AGS cell proliferation activity, and Western blotting was used to detect the effect of FMN2 on the expression of apoptosis-related protein caspase-3 in AGS cells. Results: The expression level of FMN2 in gastric adenocarcinoma tissues was significantly lower than that in matched adjacent tissues and the expression level of FMN2 was closely related to the TNM stage and differentiation of gastric adenocarcinoma (all P<0.05). Compared to AGS control group, the proliferation activity of AGS/FMN2 was significantly decreased and the expression of apoptosis-related gene Caspase-3 was markedly increased (all P<0.05). Conclusion: The expression of FMN2 was significantly decreased in gastric adenocarcinoma tissues and its low expression is closely related to the degree of tumor differentiation and clinical TNM stage. Moreover, FMN2 over-expression significantly decreased the proliferation of AGS cells. FMN2 may function as independent risk factor for the prognosis of gastric adenocarcinoma,which may provide new ideas for the treatment of gastric adenocarcinoma.
Keywords:gastric adenocarcinoma  formin-2(FMN2)  AGS cell  proliferation  apoptosis
查看全文  查看/发表评论  下载PDF阅读器

Copyright © Biother.Org™ All Rights Reserved; ISSN: 1007-385X  CN 31-1725
主管单位:中国科学技术协会 主办单位:中国免疫学会、中国抗癌学会
地址:上海市杨浦区翔殷路800号 邮政编码:200433 京ICP备13026026号-2

本系统由北京勤云科技发展有限公司设计